4. Conclusion
An UHPLC method for the determination of tea flavonol glycosides
(FOG) has been developed for the first time, and the tea sample
clean-up method by means of polyamide column chromatography
was optimized with 3 steps of elutions. With this new UHPLC
method, rutin (quercetin-3-O-rutinoside) and K-grg (kaempferol-
3-O-glucorhamnoglucoside) were successfully separated. Another
advantage of the UHPLC methods is the shorter separation time.