Fig. 5. Effect of Combretum leprosum extract and arjunolic acid on Bothrops jararaca and Bothrops jararacussu venoms activities on hemostasis.In panel A,mice received
intradermic injection(0.1mL) of physiological saline solution (PSS)or Bothrops jararaca venom (V)alone or associated with Combretum leprosum extract(Ext.)or arjunolic
acid (Arj.)in different protocols.Mice were killed 2h after venom injection,and the skin was prepared for measurement of absorbance indicating hemorrhagic activity.
In Panel B,mice tail bleeding induced by Bothrops jararaca venom (V) alone or preincubated with Combretum leprosum extract(Ext.)or arjunolic acid (Arj.).Panel C shows the time spent for mice blood collected in a capillary (previously containing venom alone or associated with treatments)to clot.Data report mean 7 SEM (n¼5). ANOVA
Dunnett’s post-hoc test.