Elongated shoots (2–4 cm) were excised and transferred to MS medium for rooting. Individual plantlets were
acclimatized in a mixture of soil and vermiculite (1:3) under culture room conditions in plastic cups covered with
transparent polythene bags with holes to control humidity by gradually increasing the holes every week for 15
days. Hardened plants were transplanted into pots containing manure and soil (1:2) under green house
conditions.