Materials andmethods: The CQhexaneextractwasfractionatedsequentiallywithbenzene,dichlor-
omethane, ethylacetate,andmethanol.TheexaminationofCQextractanditsfractionswasguidedby
bioassaysforalkalinephosphatase(ALP)activityduringthedifferentiationofMC3T3-E1osteoblastic
cells. ThecellsweretreatedwithorwithouttheCQextractanditsfractionsforaperiodoftime,andthen
the stimulatoryeffectofthealkalinephosphataseenzyme,abonedifferentiationmarker,was
investigated.Thecompoundsobtainedwerestructurallyelucidatedusingspectroscopictechniques
and re-evaluatedforactivityduringbonedifferentiation.
Materials andmethods: The CQhexaneextractwasfractionatedsequentiallywithbenzene,dichlor-omethane, ethylacetate,andmethanol.TheexaminationofCQextractanditsfractionswasguidedbybioassaysforalkalinephosphatase(ALP)activityduringthedifferentiationofMC3T3-E1osteoblasticcells. ThecellsweretreatedwithorwithouttheCQextractanditsfractionsforaperiodoftime,andthenthe stimulatoryeffectofthealkalinephosphataseenzyme,abonedifferentiationmarker,wasinvestigated.Thecompoundsobtainedwerestructurallyelucidatedusingspectroscopictechniquesand re-evaluatedforactivityduringbonedifferentiation.
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