FAN was determined using the International
Ninhydrin Colorimetric Method (Analytica EBC,
Fourth edition, 1987), while reducing sugars were
determined using Lane and Eynon’s method.
Percent alcohol in the fermenting pito samples, was
calculated and expressed as Percent Alcohol by
Volume (%ABV) using the formula:
Percent Alcohol by weight (%ABW) = (O.G – F.G) x f1
Percent Alcohol by Volume (%ABV) = %ABW x f2
Where O.G. = Original gravity
F.G. = Final gravity
f1
and f2 are factors which are 105 and 1.25
respectively