Type III. Type III resistant starch (RSIII) is retrograded amylose and starch (21–23). Because amylose molecules have
linear structures, they have a great tendency to form double
helices, particularly near refrigeration temperatures (4–58C)
and with adequate moisture content. Retrograded amylose
has high gelatinization temperatures, up to 1708C, and cannot be dissociated by cooking. The gelatinization temperature
of retrograded amylose, however, decreases with shortening of
the amylose chain length. After starchy foods are stored, particularly in a refrigerator, amylose molecules and long branch
chains of amylopectin form double helices and lose their water-binding capacity. The double helices of starch molecules
do not fit into the enzymatic binding site of amylase, thus
they cannot be hydrolyzed by this enzyme.