Cytogenetic analysis of the chromosomal variations was carried out for primary culture as well as 4-month-old callus culture. The chromosome number showed wide range of variation, however, the majority of the cells studied were observed to be diploid (2n= 16) in nature. Among the numerical observations induced, polyploid cells were the most frequent. The frequency of bridges was observed among the other structural changes. The composition and concentration of the growth regulators was found to effect chromosomal instability with multinucleate, multinucleolate and cytomixis phenomena. These observations indicated that genetic variations may arise during cell culture.