Result obtained in the treatment ‘‘Artemia with bacterial
strains PH and VA”, showed a lower survival and Artemia
nauplii did not survive for more than 4 days (Fig. 1 A and C).
These results can be explained by the negative effect of PH
because of the components of extracellular matrix (ECM) such
as fibronectin (Fn), collagen (Cn), laminin, and proteoglycans
(3, 21). In addition, the attachment of some pathogenic bacteria
like V. alginolyticus and V. parahaemolyticus to the body
surface of Artemia, causing a deleterious effect, can explain the
negative effect of V. alginolyticus (12).
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In conclusion, this study shows that Artemia culture
conditions can be controlled using Candida utilis and
Pseudomonas stutzeri. Data suggest that protection against
pathogenic bacteria depends on the nature of treatment and the
quality and quantity of potentials probiotics used. It is hoped
that more studies are required in order to clarify the exact
contribution of potential candidates probiotics used in this
study an improvement of Artemia performance and protection
against pathogens using host gene – expression and overcome
disease in aquaculture farms.