Antibiotic solutions
Antibiotics are added to mycological media to inhibit bacterial growth. Chloramphenicol is the antibiotic of choice, because it is stable under autoclave conditions. Therefore, media preparation is easier and faster due to the elimination of the filtration step. The recommended concentration of this antibiotic is 100 mg/liter medium. If bacterial overgrowth is apparent, prepare media by adding 50 mg/liter chloramphenicol before autoclaving and 50 mg/liter filter-sterilized chlortetracycline when the media have been tempered, right before pouring plates.