As the fruit were ripe, there was no need to include a DMSO step to solubilise and remove starch. Outer pericarp tissue from six fruit, about 5 g per fruit, was combined, homogenised in 80% ethanol, centrifuged, the ethanol removed and the alcohol-insoluble solids (AIS) washed a further two times with 80% ethanol, freeze dried and held over P2O5