In our laboratory we have also found the anti-staphylococcal activity of zederone, isolated from the ethanolic extract of Z. zerumbet (L.) Smith against a series of multi-drug resistant (MDR) and methicillin resistantStaphylococcus aureus strains: SA1199B, ATCC25923, XU212, RN4220 and EMRSA15[25]. These lead us to evaluate the antibacterial, MIC and antifungal activities of the ethanol extract and its different fractions of the rhizomes of Z. zerumbet (L.) Smith against thirteen pathogenic bacteria and three fungi.