Briefly, the reaction system was incubated for 5 min at 25 °C, by
sequential addition of the buffer, heme, test compounds, and Cox-1 or
Cox-2 into the system followed by mixing with TMPD and arachidonic
acid, and soft agitation for several seconds. The absorbance value was
recorded at a wavelength of 590 nm after another 15 min of incubation
at 25 °C. The performance of the assay was checked using SC-560