Amylose content was determined by the
starch-iodine blue method [15]. Milled rice
was ground into flour and then dispersed in
water. Treated with ethanol and sodium
hydroxide. The solution was heated for an
hour and allowed to remain at room
temperature overnight. The pH was then
adjusted using acetic acid and then adding a
solution of iodine. Amylose formed a
complex with iodine. Color intensity,
measured by a spectrophotometer, was then
correlated with amylose content.