A novel design of combined Sphingobacterium sp. LD-1 pretreatment
with NU pretreatment was conducted. An obvious enhancement
on enzymatic hydrolysis was achieved by combination
pretreatment, indicating a synergistic effect existed in the stepwise
pretreatment for RS saccharification. In the combination pretreatment
process, a rapid bacterial treatment by LD-1 reduced the
requirements of the subsequent NU treatment. Furthermore, combination
pretreatment with LD-1 and 4%/6% NU at 10 C resulted
in 1.396-fold and 1.372-fold increase of reducing sugar and glucose
yield respectively than that of sole NU treatment. The novel stepwise
pretreatment presents more promising for NU treatment on
industrial application.