The 236 yeasts isolates obtained from 25 fresh-cut apple samples were identified on basis of restriction fragment length
polymorphism of the ITS-5.8 rDNA region and of sequencing the
26S rRNA gene' D1/D2 region. The yeast isolates originated PCR
products ranging from 400 to 775 bp and their restriction produced
18 different RFLP profiles as shown in Table 2. Isolates in each group
ranged from 1 to 113 (Table 2). According to the results, yeast isolates
were grouped into 15 species belonging to 10 genera. Profiles I,
VI and XV did not originate D1/D2 rDNA sequences identical to any
reference species and thus were only associated with genera
Candida, Saturnispora, and Hanseniaspora, respectively