2.6. Statistical analysis
The treatments were arranged in a completely randomized design (CRD) (callus induction and rooting) and an orthogonal experimental design (plant regeneration). All experiments were repeated three times, and each treatment contained twenty explants. The data were subjected to analysis of variance (ANOVA),and a significance comparison among the means was performedusing Duncan’s multiple-range test; a P value of less than 0.05 was considered significant.