The two strawberry cultivars grew well in In Lunar Palace 1, their leaves being luxuriant and inflorescences massive. Fruit harvested in In Lunar Palace 1 was similar to that in field conditions, bright red in the color, and round in the shape and delicious tasted. The production data of the two cultivars during the experiment is shown in Table 1, with a total of 70 days of harvest. The average supply of fresh berries for the experimental volunteers was 37.4 g daily. The plant of ‘Frandy’ cultivars grew better than ‘Benihoppe’ and better adapted to a closed system. Meanwhile, the yield of ‘Frandy’ was significantly higher (nearly twice) than ‘Benihoppe’, and the harvest index of ‘Frandy’ was 0.32, also much higher than that of ‘Benihoppe’ (0.18). The low harvest index of ‘Benihoppe’ may be due to inappropriate environment which had certain influence on blossoming, fruit bearing, and the growth of stolon which led to an increase in inedible biomass. High yield and high ratio of edible part are important principles of plant screening for life support system (Krauss, 1962 and Wheeler et al., 1996). The harvest index of ‘Frandy’ was similar to that of strawberry in Advanced Life Support experiment by NASA (0.3–0.4) (Wheeler and Sager, 2006). Considering the above mentioned, ‘Frandy’ is better to be cultivated in a closed life support system.