The input/output plasmid is the plasmid with the rhamnose promoter, the CIλ repressor, CItet promoter and the GFP or toxins see figure 5 and 6. It is assembled with the standard BioBrick assembly method. The end product is assembled into a low copy number plasmid to reduce the metabolic stress, enable faster reactions to input repressors, lower the GFP production so that it can be measured more accurately and to reduce the possibility that leakiness in toxin production will kill the cell. Another low copy plasmid than pSB3K3 can also be used.