During soy sauce fermentation, most of the proteins from soybeans
and wheat were primarily hydrolyzed into short peptides
or free amino acids (Lioe, Wada, Aoki, & Yasuda, 2007). Soy sauce
was separated and enriched by XAD-16 resin. The column was
sequentially eluted with deionized water, 20% (v/v) ethanol, 40%
(v/v) ethanol and 60% (v/v) ethanol. Consequently, soy sauce was
separated into XAD-0%, XAD-20% and XAD-40% fractions. The elution
processes was obvious different between column chromatography
and static absorption/desorption on resins. In the elution of
column chromatography studies, most of the water-soluble material
in soy sauce was enriched in the fractions of low concentration
of ethanol, in contrast, few material was in the 60% ethanol fraction.
While about static absorption and desorption on resins, only
one concentration of ethanol was acted on the material which
absorbed on the resins, so the 60% ethanol fraction enriched some
of the material of soy sauce. The amounts of the total amino acids
and amino acids in the peptide of different fractions after the treatment
with gradient elution on XAD-16 resin column are listed in
Table 5. The contents of total umami amino acids of fractions were
During soy sauce fermentation, most of the proteins from soybeansand wheat were primarily hydrolyzed into short peptidesor free amino acids (Lioe, Wada, Aoki, & Yasuda, 2007). Soy saucewas separated and enriched by XAD-16 resin. The column wassequentially eluted with deionized water, 20% (v/v) ethanol, 40%(v/v) ethanol and 60% (v/v) ethanol. Consequently, soy sauce wasseparated into XAD-0%, XAD-20% and XAD-40% fractions. The elutionprocesses was obvious different between column chromatographyand static absorption/desorption on resins. In the elution ofcolumn chromatography studies, most of the water-soluble materialin soy sauce was enriched in the fractions of low concentrationof ethanol, in contrast, few material was in the 60% ethanol fraction.While about static absorption and desorption on resins, onlyone concentration of ethanol was acted on the material whichabsorbed on the resins, so the 60% ethanol fraction enriched someof the material of soy sauce. The amounts of the total amino acidsand amino acids in the peptide of different fractions after the treatmentwith gradient elution on XAD-16 resin column are listed inTable 5. The contents of total umami amino acids of fractions were
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