We developed a simultaneous saccharification and fermentation (SSF) process of cassava flour at very
high gravity (VHG). Cassava flour (CF) was dissolved in water to reach 315.4 g/l dry matter, and then the
mixture was liquefied at 80 ◦C for 90 min by using alpha-amylase (3532AAU/kg CF) and beta-glucanase
(2812 U/kg CF). SSF of liquefied mash of cassava was performed at 30 ◦C with the simultaneous addition of
two glucoamylases (Distillase ASP at 540 GAU/kg CF and Amigase Mega L at 0.035% w/w), active dry yeast
(1.5 × 107 cells/l), urea (12 mM) and KH2PO4 (4 mM). Under these conditions, the SSF process finished
after 72 h. The ethanol content achieved 17.2% v/v corresponding to 86.1% of the theoretical ethanol yield
at lab scale and decreased to 16.5% v/v corresponding to 83.6% of the theoretical ethanol yield at pilot
scale. Therefore, the SSF of cassava flour under VHG condition could have a great potential for the ethanol
industry in Vietnam and South East Asia.