Two 0.5-mL aliquots
of raw semen were prepared; 1 aliquot was placed in a
16°C cooler until analysis for concentration, and the second
aliquot was frozen in liquid nitrogen and maintained
at −80°C for later assessment of DNA quality using the
sperm chromatin structure assay (SCSA). Raw semen
was also preserved in buffered formol saline for pending
morphological analysis