3.4. Diurnal rhythms of digestive enzyme activities
3.4.1. Lipase activity
The diurnal rhythm of lipase activity during the 24-h cycle was similar for all sizes of sea cucumbers (P N 0.05; Table 3). Lipase activity was highest at 00:00 and was relatively lower from 14:00 to 18:00 (Fig. 7A). Sea cucumbers of different sizes showed no difference in lipase activity during the diurnal cycle but significant differences were found among different times for all three sizes of sea cucumbers (Table 3).