The liquid
MS medium (0.5 mL) containing 0.4 M sucrose was then added and
held for 10 min before removal and the process was repeated again.
One milligram of the liquid MS medium containing 0.4 M sucrose
was added next and held for 10 min, and then removed. Finally, a
liquid MS medium containing 0.02% Phytagel and 58 mM sucrose
was added and held for 3 d at 25 ◦C in the dark.