A total of 40 double-muscled Belgian Blue young bulls with an
average age and carcass weight at slaughter of 21.6±2.7 months
and 502.4±42.2 kg respectively were used. Animals originated
from 16 different farms and were slaughtered in 4 commercial abattoirs
on 5 days. Animals were slaughtered and cooled according to
common practices, i.e. stunned by captive bolt, shackled and bled
immediately, dehided, eviscerated, split and sent to a conventional
chilling room after approximately 1 hour.
At 48 hours post-mortem, a slice of 2.5 cm thickness of the LD was
taken at the height of the 7th rib. At the thickest part of the hind leg a
slice of 2.5 cm thickness was taken from the M. biceps femoris (BF).
The inner and outer BF sample (IBF and OBF) were 6 cm width and
were taken from the long head of the BF. The IBF and OBF sample
were taken from the most interior and the most exterior part respectively.
The middle part was not further used. A first set of subsamples
was vacuum packed and frozen at −20 °C until enzyme activity and
α-tocopherol analysis. For the simulated retail display, a second set
of subsamples was wrapped in oxygen permeable foil and continuously
displayed at 4 °C under fluorescent light (approximately
1200 lux) for 10 days
A total of 40 double-muscled Belgian Blue young bulls with anaverage age and carcass weight at slaughter of 21.6±2.7 monthsand 502.4±42.2 kg respectively were used. Animals originatedfrom 16 different farms and were slaughtered in 4 commercial abattoirson 5 days. Animals were slaughtered and cooled according tocommon practices, i.e. stunned by captive bolt, shackled and bledimmediately, dehided, eviscerated, split and sent to a conventionalchilling room after approximately 1 hour.At 48 hours post-mortem, a slice of 2.5 cm thickness of the LD wastaken at the height of the 7th rib. At the thickest part of the hind leg aslice of 2.5 cm thickness was taken from the M. biceps femoris (BF).The inner and outer BF sample (IBF and OBF) were 6 cm width andwere taken from the long head of the BF. The IBF and OBF samplewere taken from the most interior and the most exterior part respectively.The middle part was not further used. A first set of subsampleswas vacuum packed and frozen at −20 °C until enzyme activity andα-tocopherol analysis. For the simulated retail display, a second setof subsamples was wrapped in oxygen permeable foil and continuouslydisplayed at 4 °C under fluorescent light (approximately1200 lux) for 10 days
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