Most tissue culture studies in the Orchidaceae family have re- ported that SEs were derived from the epidermal cells of mother tissue (Chen and Hong, 2012; Cueva-Agila et al, 2016). However, we found that the SEs originated from the epidermal and the subepidermal cells of explant. This result also reported in C. bicolor (Mehendran and Bai, 2012) and D. crumenatum (Kaewubon and Meesawat, 2016). Although the process of SEs formation has occured earlier at the cellular level, but visually SES were seen as a proembryo mass that rapidly differentiated to formed a globular- shape embryo, This embryo undergo differentiation by formed a terminal notch. The notch marks the future location in the shoot apex and represents the earliest morphological sign of coleoptile development (Alcantara et al. 2014). At this stage, cell divisions were most frequent in the terminal cells that eventually forms shoot and root meristem. Matsumoto et al. (1996) mentioned that the formation of these features designated the bipolarity of SE and fulfill the anatomical requirements of a true SE. In conclusion, NP medium supplemented with 3 mgl TDZ was the best medium to induce direct SEs from all various explants of P. amabilis. While protocorm and stem appeared to be the most responsive explants due to the fastest and the highest number of SEs produced. The easy procedure for SEs production using TDZ- containing medium will support and give high benefit for orchid conservation as well as orchid industry.