The
substrates of reactors T5, T6 and CK1 were incubated with the
cellulase (Trichoderma Viride, produced by Shaihai boao biotechnology
co. Ltd) solution at 40 ◦C in an orbital shaker (150 r min−1)
for 72 h; but the substrates of other reactors were incubated with
the filter-sterilized enzyme solution at the same condition, and
aseptic conditions were maintained throughout the experiments;
600 mL of the total test material was added to each reactor (Table 2).
Experiments of all reactors were performed in triplicate and the
enzyme loading was 40 A.U. g−1 VS (volatile solid) fed according
to the former study [22]