The antioxidant activity of the extract was measured with the
DPPH method [18] with slight modifications. A solution of
DPPH was freshly prepared by dissolving 6 mg DPPH in 50 mL
methanol (about 0.3 mM). The extract (2.5 mL) with varying
concentrations (60e220 mg/mL) and DPPH solution (2.5 mL)
was mixed together in a test tube. The test tube was then
incubated in the dark for 20 minutes at room temperature.
The decrease in absorbance was measured at 517 nm using a
UVeVIS spectrophotometer.