The study demonstrates that dietary supplementation of biofloc at 4–8% level had beneficial effects on growth performance and digestive enzyme activities in P. monodon. Current method of biofloc production using ammoniumsulphate as nitrogen and wheat flour as carbon source is cheaper and easier compared to bioreactors. These findings may encourage feed manufacturers to consider biofloc as a viable alternative dietary supplement. Future studies are required to determine the amino acid profile,mineral profile and non-protein content of the biofloc with respect to nutritional requirement of shrimp.