2.4. Preparation of the culture media
The concentrated soybean molasses received from local
industries with 550e650 g L-1 of soluble solids was diluted
with distilled water to produce the culture media with the
desired sugar concentration, without any supplementation of
other nitrogen or carbon sources, or salts. The total soluble
solids concentration was determined with the aid of
a saccharimeter. The pH was adjusted to the desired value
with 1000 mol.m-3 HCl or 3000 mol.m-3 NaOH, depending
on the case. The material was sterilized at 121 C during
15 min