The purity of the enzyme was determined by SDS-PAGE in Tris-glycine buffer. Fractions containing the His6- tagged protein were pooled, dialyzed against phosphate buffer (50 mM, pH 7) containing 300 mM NaCl, and analyzed for esterase activity.
The purity of the enzyme was determined bySDS-PAGE in Tris-glycine buffer. Fractions containing the His6-tagged protein were pooled, dialyzed against phosphate buffer(50 mM, pH 7) containing 300 mM NaCl, and analyzed for esteraseactivity.