The separation was achieved within 30 min by the application of high voltage in the sieving polymer and specialized buffer in the microfluidic channels through independent electrodes for each well and the automated electrophoretic patterns as shown in Fig. 3 of lane 1–5 showed a single band of targeted PCR products from cat, pig, pork, monkey and rat meat species, respectively, without producing any fragment of non-specific amplification, indicating high species-specificity of the primers towards the target species.