Filter paper was placed in 50 cc Iso glass and about 10 cc of 0.1% solution of autoclaved DEPC was added to the Iso glass. Filter was properly washed three times with the help of sampler in the Iso glass wall through immersion and extreme turbulence. Then the solution resulted from washing were transferred into centrifuge Falcon tubes. Then, the solution was centrifuged for 10 min with 14 000 r/min.