The iodine value method is reproducible only if the exact conditions of the test are carefully followed. Any changes in strength of reagent, sample size or reaction time may produce varying results.
The exact specified weight of the sample is accurately weighed into a glass stoppered iodine flask, and dissolved in chloroform. The measured volume of Hanus reagent is accurately added and after thorough mixing, is placed in the dark for exactly one hour. A corresponding reagent blank is simultaneously prepared.