only a few reports on LAB cultures are available. Investigations suggest that LAB are relatively
resistant to the toxic effect of spices, furthermore, some exert a stimulatory effect on these organisms (Zaika & Kissinger, 1981). Marhamatizadeh et al. (2012) obtained probiotic milk and yoghurt using two LAB strains (Lactobacillus acidophilus and Bifidobacterium
bifidus) and observed that oregano stimulated their growth and acid production. The presence of 0.5e1.0 g/l of oregano increased 2.5 times the TTA and cell counts were higher than control without the spice during 7 days of fermentation, but increasing concentrations progressively delayed bacterial growth and acid production. On the other hand, Kivanc, Akgul, and Dogan (1991) reported that 0.5% of oregano inhibited growth of two strains of Leuconostoc mesenteroides and Lactobacillus plantarum, although acid production by L. plantarum was stimulated. Therefore, inhibitory or stimulatory effects of spices are strain-specific and also depend on their concentration. In this case, Potato Cheese (CH puree þ 1.5% NaCl þ 0.8% oregano) had slight but significantly (P < 0.05) higher values of viable cells than the puree without additives during 72 h of fermentation, indicating that oregano used in the concentration analyzed may stimulate metabolic activities of L. brevis CJ25.
only a few reports on LAB cultures are available. Investigations suggest that LAB are relativelyresistant to the toxic effect of spices, furthermore, some exert a stimulatory effect on these organisms (Zaika & Kissinger, 1981). Marhamatizadeh et al. (2012) obtained probiotic milk and yoghurt using two LAB strains (Lactobacillus acidophilus and Bifidobacteriumbifidus) and observed that oregano stimulated their growth and acid production. The presence of 0.5e1.0 g/l of oregano increased 2.5 times the TTA and cell counts were higher than control without the spice during 7 days of fermentation, but increasing concentrations progressively delayed bacterial growth and acid production. On the other hand, Kivanc, Akgul, and Dogan (1991) reported that 0.5% of oregano inhibited growth of two strains of Leuconostoc mesenteroides and Lactobacillus plantarum, although acid production by L. plantarum was stimulated. Therefore, inhibitory or stimulatory effects of spices are strain-specific and also depend on their concentration. In this case, Potato Cheese (CH puree þ 1.5% NaCl þ 0.8% oregano) had slight but significantly (P < 0.05) higher values of viable cells than the puree without additives during 72 h of fermentation, indicating that oregano used in the concentration analyzed may stimulate metabolic activities of L. brevis CJ25.
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