Cells were cultivated at 37 C in lysogeny broth (LB) medium with 50 lgml–1 Kanamycin until an optical density of between 0.6 and 0.8 at 600 nm was reached.
Cells were cultivated at 37 C in lysogeny broth (LB) medium with 50 lgml–1 Kanamycinuntil an optical density of between 0.6 and 0.8 at 600 nm was reached.