The KINV slightly increased while KTP considerably decreased with an increase in volume ratio (Fig 6). The enzyme was found to be getting concentrated in the bottom
phase (preferred partitioning phase) with an increase in volume ratio due to the reduction of its volume. The low enzyme recovery observed at high volume ratio (>2.28) was due to the precipitation (as the bottom phase reaching its solubility limit). Even though highest activity of 109.4 U/ml was observed at volume ratio of 3.6, it resulted in lower recovery 74.43% and purification (5.67) compared to those at volume ratio of 2.28. The highest activity recovery of
91.99% was observed with maximum purification of 6.69 fold was observed at volume ratio of 2.28.