The development in the peptide profile of Milk72 (Fig. 8)
showed a rapid increase of peaks with 31e34 min retention time
between 0 and 4 weeks storage. These peaks were identified as proteose peptones. After 8 weeks storage, more peptides appeared between 17 and 30 min. Most of the peptides eluted late in the RPUHPLC, which has been reported previously to correlate with PL activity (Datta & Deeth, 2003). The peptide profile changed slightly
between 12 and 14 weeks storage, although the total peak area of the peptide profile of Milk72 remained constant in the same time period. The most profound change in the peptide profile between 12 and 14 weeks storage was seen in the proteose peptone peaks.The proteose peptone -casein A1f(1e105) eluting at 33.0 min decreased and -casein A1
f(29e105) eluting at 32.7 min increased (peptides are indicated by arrows in Fig. 8). This shows that PL was still active after gelation of Milk72, as already suggested by the PL