Mean recoveries of cyprohepta-dine from four kinds of feeds spiked at 0.1, 1.0 and 10 mg kg-1ranged from 85.5% to 96.2%, with intra-dayand inter-day relative standard deviation less than 10%. The calibration curve of cyproheptadine was good linear relationship (r > 0.9993) within the range of 0.1–50 g mL-1. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 and 0.1 mg kg-1, respectively.