Recently, Zhao et al., using a scaffold with CPC and
chitosan fibers, harvested human umbilical cord mesenchymal
stem cells (hUCMSCs) without an invasive
procedure that is commonly required when studying bone
marrow mesenchymal stem cells (MSCs). The objectives
were to develop CPC scaffolds with improved resistance
to fatigue and fracture, for the delivery of hUCMSC aimed
at bone tissue engineering. In “fast fractures”, CPC with
15% chitosan and 20% polyglactin [(C2H2O2)m(C3H4O2)n]
fibers (CPC–chitosan–fiber scaffold) had flexural strength
of 26 MPa, while CPC control was 10 MPa. The hUCMSCs
showed excellent viability when seeded in CPC and
CPC–chitosan–fiber scaffolds, the live cell retention was
96–99%. The cell density was about 300 cells/mm2 on day
1; and with proliferation ∼700 cells/mm2 at day 4 (Fig. 12).
Wst-1 assay showed that the stronger CPC–chitosan–fiber
scaffold had hUCMSC viability thatmatched the CPC control
(p > 0.1). This study indicated that chitosan and polyglactin
fibers substantially increased the fatigue resistance of CPC,
and that hUCMSCs had excellent proliferation and viability
on the scaffolds [230]