Great care was taken not to puncture the
gut or muscle somites during the hemolymph extraction. Osmolality
of a sample was immediately measured with a vapor pressure
osmometer (Wescor® Vapro 5520). Replicate samples from each
amphipod were averaged. Water samples were taken directly from
the acclimations solutions, and the average osmolality was calculated
from a minimum of three replicate measurements. All osmolality data
was expressed in milliosmoles per kilogram (mOsm kg−1).