PCR wasperformed in 10 μL reactions containing 5–25 ng of DNA template, 1 μL 10X TB buffer (containing 200mM Tris-
HCl pH 8.3, 500mM KCl, 15mM MgCl2), 1 μL of 1mM dNTP, 0.50 μL each of 5μM forward and reverse primers,
and 0.25 μL of Taq DNA polymerase (4U/μL) using an MJ Research single or dual 96-well thermal cycler