The inoculum was prepared as follows: to 100 ml of the Kirk’s media the following were added: glucose, 10 g l
1
; ammonium nitrate, 2 g l
1
; corn steep liquor (CSL), 2 g l
1
; yeast extract, 2 g l
. Then 20 mycelial discs (0.5 cm from 7-day culture) from potato dextrose broth (PDB) in petri dishes were taken and incubated for 7 days at 24
C on a rotary shaker at 160 rpm.