Reduction of
the strength of macro- and micro-elements in the basal
medium from half to one-third, with addition of 0.49 lM
indole-3-butyric acid and 0.05% activated charcoal, and
placing the cultures under reduced light intensity
(17 lmol m-2 s-1), was found to alleviate chlorosis and
improve micropropagation with high quality Rubus plantlets.
Response to in vitro culture differed greatly and
consequently different methods of Micropropagation were
required by different Genotypes