AmB extracts all the sterols from the membranes,which are incorporated in the AmB sponge layer attached to the surface of themembrane. Most notably, the sterol depletion has a significant thinning effect on the naturally polyunsaturated membranes containing ergosterol, in which
case the hydrophobic thickness decreases by 20%, whereas only a modest thinning is observed in less polyunsaturated membranes. Thus, the membrane fatty acid composition seems to be an essential parameter governing the structural modification induced by both AmB insertion [22,25] and ergosterol removal