The human colon cancer cell line HCT 116 was cultured in RPMI-1640 medium supplemented with 25 mM HEPES buffer, 25 mM
sodium bicarbonate, 300 mML-glutamate and 10% heat inactivated
fetal bovine serum (Gibco USA) in 5% CO2incubator 37C. For the
experiments 3 ml cell culture was plated at 1x105 cells/plate confluency