We cut the shoot in such a way that each piece of 1.5cm long shoot contained two axillary buds and cultured
them into the initiation media. We used ½, ¾ and standard MS media to check optimal response. After 3-4 days,
most prominent induction of leaves was observed in ¾ MS. Leaves form each bud were emerged and continues
to grow during first week. After two weeks, one prominent shoot of 4 cm was emerged from each bud having a
number of leaves. At the end of 3rd week, the shoot length increased up to 4.5 cm. The explants were kept in the
same media for three weeks until the shoot reaches a length of 4cm. As initiation media is ¾ MS without
hormone, it means that initiation of Phaius tankervilliae does not require any hormone. Whereas, the control
plant was set by planting a 10 cm long leafless shoot of Phaius tankervilliae in the soil. The induction of leaves
could not be observed before twenty days. It suggests that ¾ MS media has enough potential to stimulate rapid
initiation in Phaius tankervilliae.