micro-centrifuged 1 min at 10,000 rpm (Scientific model V; VWR, Bristol, CT, USA), filtered
0.45 lmsyringe filter, dispensed into clean micro-centrifuge tubes, and retained frozen until HPLC analysis.
micro-centrifuged 1 min at 10,000 rpm (Scientific model V; VWR, Bristol, CT, USA), filtered0.45 lmsyringe filter, dispensed into clean micro-centrifuge tubes, and retained frozen until HPLC analysis.
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