A total of 67 LAB, including S. thermophilus, were isolated from
traditional home-made yogurt samples randomly collected
from different region of Turkey in a previous study (Gezginc,
2010). Isolated LAB were identified using biochemical tests and
sequencing. The 16S rRNA region PCR amplification and sequence
analysis of strains were performed according to the methods of
Tabasco, Paarup, Janer, Pelaez, and Requena (2007). A total of 58
S. thermophilus strains were examined. S. thermophilus strains were
routinely grown at 42 C in SM17 medium (Merck, Germany).