Flow cytometry
The ploidy levels of the control and regenerated plants were analyzed by flow cytometry. Rosette leaves were chopped and a suspension of nuclei was isolated with an extraction buffer (Dolezel et al. 1998). The samples were filtered through a 30 lm nylon mesh (Partec Cell Trics)
and stained with DAPI (40-6-diamino-2-phenyloindole).The measurements were performed with the use of a DAKO Galaxy flow cytometer equipped with an HBO-100 mercury lamp and an argon ion laser.