One milliliter of extracts or fractions with different concentrations was mixed with 2.5 ml of phosphate buffer (200 mM; pH 6.6) and 2.5 ml of potassium ferricyanide1% and incubated at 50oC for 20 min. The mixture was added with 2.5 ml of 10% TCA and centrifuged at 3000 rpm for 10 min. A-2.5 ml of supernatant was mixed with 2.5 ml of distilled water and 0.5 ml of FeCl3 (0.1%) and the absorbance was measured spectrophotometrically at 700 nm. Increase in absorbance of the reaction mixture was interpreted as increase in reducing activity of the extract and the results were compared with ascorbic acid which was used as a positive control. The percentage of reduction of the sample as compared to standard (ascorbic acid) was calculated using the formula: